[Molecular cloning and characterization of two oxidative stress related genes from medicinal fungus Polyporus umbellatus].
Identifieur interne : 000233 ( Main/Exploration ); précédent : 000232; suivant : 000234[Molecular cloning and characterization of two oxidative stress related genes from medicinal fungus Polyporus umbellatus].
Auteurs : Mengmeng Liu ; Chao Song ; Yongmei Xing ; Shunxing GuoSource :
- Wei sheng wu xue bao = Acta microbiologica Sinica [ 0001-6209 ] ; 2015.
Descripteurs français
- KwdFr :
- Alcohol oxidoreductases (composition chimique), Alcohol oxidoreductases (génétique), Alcohol oxidoreductases (métabolisme), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Masse moléculaire (MeSH), Peroxidases (composition chimique), Peroxidases (génétique), Peroxidases (métabolisme), Phylogenèse (MeSH), Point isoélectrique (MeSH), Polyporus (classification), Polyporus (composition chimique), Polyporus (enzymologie), Polyporus (génétique), Protéines fongiques (composition chimique), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Stress oxydatif (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Alcohol oxidoreductases, Peroxidases, Polyporus, Protéines fongiques.
- enzymologie : Polyporus.
- génétique : Alcohol oxidoreductases, Peroxidases, Polyporus, Protéines fongiques.
- métabolisme : Alcohol oxidoreductases, Peroxidases, Protéines fongiques.
- Clonage moléculaire, Données de séquences moléculaires, Masse moléculaire, Phylogenèse, Point isoélectrique, Stress oxydatif, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Alcohol Oxidoreductases (chemistry), Alcohol Oxidoreductases (genetics), Alcohol Oxidoreductases (metabolism), Amino Acid Sequence (MeSH), Cloning, Molecular (MeSH), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Isoelectric Point (MeSH), Molecular Sequence Data (MeSH), Molecular Weight (MeSH), Oxidative Stress (MeSH), Peroxidases (chemistry), Peroxidases (genetics), Peroxidases (metabolism), Phylogeny (MeSH), Polyporus (chemistry), Polyporus (classification), Polyporus (enzymology), Polyporus (genetics).
- MESH :
- chemical , chemistry : Alcohol Oxidoreductases, Fungal Proteins, Peroxidases.
- chemical , genetics : Alcohol Oxidoreductases, Fungal Proteins, Peroxidases.
- chemical , metabolism : Alcohol Oxidoreductases, Fungal Proteins, Peroxidases.
- chemistry : Polyporus.
- classification : Polyporus.
- enzymology : Polyporus.
- genetics : Polyporus.
- Amino Acid Sequence, Cloning, Molecular, Isoelectric Point, Molecular Sequence Data, Molecular Weight, Oxidative Stress, Phylogeny.
Abstract
OBJECTIVE
To clone the NADPH gene (PuNOX) and Glyoxal oxidase gene (PuGLOX) from a medicinal fungus Polyporus umbellatus, and to carry out the bioinformatic analysis.
METHODS
We used the Rapid Amplification of cDNA ends (RACE) technique to obtain the full length cDNA of these two genes. We used a series of bioinformatic tools to characterize physiochemical properties of the two deduced protein. The analyses of multiple alignment and phylogenetic trees were performed using Bioeditor and MEGA 5.0 softwares.
RESULTS
The entire cDNA of PuNOX and PuGLOX were 1674 bp, 1723 bp in length and encoded a 557-amino acid protein and 515-amino acid protein with a molecular weight of 63.845 kDa and 55.891 kDa and the isoelectric point of 5.58 and 4.82, respectively. PuNOX had high identities (74 to 80%) with NADPH peroxidase from other fungus. From the evolutionary tree, PuNOX was closely related to that of Pleurotus ostreatus. PuGLOX had high identities (> 50%) with Glyoxal oxidases from various fungus. Phylogenetic tree analysis suggested that PuGLOX was closely related to that of Phanerochaete chrysosporium.
CONCLUSION
Molecular characterization of the two oxidative stress related genes will be useful for further functional determination of the genes involved in the sclerotium development of Polyporus umbellatus.
PubMed: 26939456
Affiliations:
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(genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alcohol oxidoreductases (composition chimique)</term><term>Alcohol oxidoreductases (génétique)</term><term>Alcohol oxidoreductases (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Masse moléculaire (MeSH)</term><term>Peroxidases (composition chimique)</term><term>Peroxidases (génétique)</term><term>Peroxidases (métabolisme)</term><term>Phylogenèse (MeSH)</term><term>Point isoélectrique (MeSH)</term><term>Polyporus (classification)</term><term>Polyporus (composition chimique)</term><term>Polyporus (enzymologie)</term><term>Polyporus (génétique)</term><term>Protéines fongiques (composition chimique)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Stress oxydatif (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" 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xml:lang="en"><term>Polyporus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Polyporus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alcohol oxidoreductases</term><term>Peroxidases</term><term>Polyporus</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alcohol oxidoreductases</term><term>Peroxidases</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Isoelectric Point</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Oxidative Stress</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Masse moléculaire</term><term>Phylogenèse</term><term>Point isoélectrique</term><term>Stress oxydatif</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>To clone the NADPH gene (PuNOX) and Glyoxal oxidase gene (PuGLOX) from a medicinal fungus Polyporus umbellatus, and to carry out the bioinformatic analysis.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>We used the Rapid Amplification of cDNA ends (RACE) technique to obtain the full length cDNA of these two genes. We used a series of bioinformatic tools to characterize physiochemical properties of the two deduced protein. The analyses of multiple alignment and phylogenetic trees were performed using Bioeditor and MEGA 5.0 softwares.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The entire cDNA of PuNOX and PuGLOX were 1674 bp, 1723 bp in length and encoded a 557-amino acid protein and 515-amino acid protein with a molecular weight of 63.845 kDa and 55.891 kDa and the isoelectric point of 5.58 and 4.82, respectively. PuNOX had high identities (74 to 80%) with NADPH peroxidase from other fungus. From the evolutionary tree, PuNOX was closely related to that of Pleurotus ostreatus. PuGLOX had high identities (> 50%) with Glyoxal oxidases from various fungus. Phylogenetic tree analysis suggested that PuGLOX was closely related to that of Phanerochaete chrysosporium.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>Molecular characterization of the two oxidative stress related genes will be useful for further functional determination of the genes involved in the sclerotium development of Polyporus umbellatus.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26939456</PMID><DateCompleted><Year>2016</Year><Month>03</Month><Day>24</Day></DateCompleted><DateRevised><Year>2016</Year><Month>03</Month><Day>04</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-6209</ISSN><JournalIssue CitedMedium="Print"><Volume>55</Volume><Issue>10</Issue><PubDate><Year>2015</Year><Month>Oct</Month><Day>04</Day></PubDate></JournalIssue><Title>Wei sheng wu xue bao = Acta microbiologica Sinica</Title><ISOAbbreviation>Wei Sheng Wu Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Molecular cloning and characterization of two oxidative stress related genes from medicinal fungus Polyporus umbellatus].</ArticleTitle><Pagination><MedlinePgn>1284-90</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To clone the NADPH gene (PuNOX) and Glyoxal oxidase gene (PuGLOX) from a medicinal fungus Polyporus umbellatus, and to carry out the bioinformatic analysis.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">We used the Rapid Amplification of cDNA ends (RACE) technique to obtain the full length cDNA of these two genes. We used a series of bioinformatic tools to characterize physiochemical properties of the two deduced protein. The analyses of multiple alignment and phylogenetic trees were performed using Bioeditor and MEGA 5.0 softwares.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The entire cDNA of PuNOX and PuGLOX were 1674 bp, 1723 bp in length and encoded a 557-amino acid protein and 515-amino acid protein with a molecular weight of 63.845 kDa and 55.891 kDa and the isoelectric point of 5.58 and 4.82, respectively. PuNOX had high identities (74 to 80%) with NADPH peroxidase from other fungus. From the evolutionary tree, PuNOX was closely related to that of Pleurotus ostreatus. PuGLOX had high identities (> 50%) with Glyoxal oxidases from various fungus. Phylogenetic tree analysis suggested that PuGLOX was closely related to that of Phanerochaete chrysosporium.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Molecular characterization of the two oxidative stress related genes will be useful for further functional determination of the genes involved in the sclerotium development of Polyporus umbellatus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Mengmeng</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Chao</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Xing</LastName><ForeName>Yongmei</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Shunxing</ForeName><Initials>S</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType 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UI="D007526" MajorTopicYN="N">Isoelectric Point</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055492" MajorTopicYN="N">Polyporus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26939456</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Guo, Shunxing" sort="Guo, Shunxing" uniqKey="Guo S" first="Shunxing" last="Guo">Shunxing Guo</name><name sortKey="Liu, Mengmeng" sort="Liu, Mengmeng" uniqKey="Liu M" first="Mengmeng" last="Liu">Mengmeng Liu</name><name sortKey="Song, Chao" sort="Song, Chao" uniqKey="Song C" first="Chao" last="Song">Chao Song</name><name sortKey="Xing, Yongmei" sort="Xing, Yongmei" uniqKey="Xing Y" first="Yongmei" last="Xing">Yongmei Xing</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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